Fig. 5

E2F8 is a direct target of miR-144. a Venn diagram showing miR-144 targets E2F8 by three prediction software packages. b Predicted miR-144 target sequences in E2F8 3’-UTR. Six nucleotides (red) were mutated to prevent binding to miR-144. c Relative luciferase activity of reporter plasmids carrying wild-type or mutant E2F8 3’-UTR in BCPAP and TPC-1 cells co-transfected with miR-NC or miR-144 mimic. Data were presented as mean ± SD. d qRT-PCR. Level of E2F8 mRNA after miR-NC or miR-144 transfected into PTC cells. e Western blot. Level of E2F8 protein after miR-NC or miR-144 transfected into PTC cells. *p < 0.05, ***p < 0.001