Fig. 6

miR-144 inhibits PTC cells proliferation via inducing G1-phase arrest. a and b Transfection of miR-144 mimic inhibited cells proliferation, while transfection of miR-144 inhibitor promoted cells proliferation in BCPAP and TPC-1 cells. c and d Colony formation ability was also decreased in both BCPAP and TPC-1 cells after they were transfected with miR-144 compared with miR-NC. e and f miR-144 significantly caused G1-phase arrest in both BCPAP and TPC-1 cells compared with miR-NC. g Effect of enforced overexpression of E2F8 could partially reverse the miR-144-mediated inhibition of proliferation in TPC-1 cells. h and i Flow-cytometry analysis showed that enforced oe-E2F8 treatment could significantly alleviate miR-144-mediated G1-phase arrest of TPC-1 cells. j E2F8 and CCND1 protein levels were determined by western blot in each group. *p < 0.05, **p < 0.01, ***p < 0.001