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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: PIG3 promotes NSCLC cell mitotic progression and is associated with poor prognosis of NSCLC patients

Fig. 2

Loss of PIG3 leads to increased outcome of bi-/multi-nucleated cells and aberrant chromosomes segregation in normal cultured cells. a Western blot analysis demonstrating the efficacy of two different siRNAs against PIG3 in A549 cells at 48 h post-transfection. b 3× 103 cells were seeded in 96-well plates at day 0, and CCK8 assay was used to determine cell proliferation rates at indicated days (1, 2, 3, 4 and 5 days). Absorbance values at 450 nm were normalized by the value measured on day 1 (** P < 0.01). c Representative images showing bi- and multinucleated cells (arrowheads). d Quantitative analysis of bi- and multi-nucleated cells from PIG3-deficient and control A549 cells (** P < 0.01). e Representative images showing normal and aberrant anaphase cells with lagging chromosome (arrowheads). f Percentages of mitotic cells showing lagging chromosome were counted from three independent experiments, ** P < 0.01, * P < 0.05

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