Fig. 2

NS3 is responsible for the regulation of PPM1A abundance. a–c Huh-7 cells were transiently transfected with plasmid encoding one of the indicated HCV proteins (a), Flag-vector or Flag-NS3 plasmids at different concentrations as indicated (b), and NS3 or NS3 S139A plasmids (c) for 48 h and subsequently collected for western blotting. d Huh-7 cells were transfected with GFP-vector, GFP-NS3 or GFP-protease for 48 h and subsequently stained for PPM1A (red); the intracellular localization of PPM1A was examined by fluorescence microscopy