Fig. 3

NS3 protein interacts with PPM1A. a–c Huh-7 cells were transiently transfected with Flag-NS3 or vector (a); HEK293T cells were cotransfected with Flag-PPM1A and GFP-NS3 (b). HEK293T cells were cotransfected with Flag-PPM1A and GFP-protease or GFP-helicase (c), 36 h later, co-IP was performed to identify the interaction between PPM1A and NS3 or its deletion mutants