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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Curcumin suppresses gastric tumor cell growth via ROS-mediated DNA polymerase γ depletion disrupting cellular bioenergetics

Fig. 2

Mitochondrial respiration and aerobic glycolysis are suppressed in response to curcumin. a. Effects of curcumin on real-time mitochondrial oxygen consumption rate (OCR). SGC-7901 and BGC-823 cells treated with 10 μg/mL curcumin for 12h were evaluated for mitochondrial oxygen consumption using the Seahorse XF96 analyzer (Methods) after sequential injection (arrows) of the ATP synthase inhibitor oligomycin (1 μM), uncoupler FCCP (1 μM), complex I inhibitor rotenone (1 μM) and complex III inhibitor antimycin A (1 μM); n = 6. Effects of curcumin (10 μg/ml for 12 h) on b. cellular basal respiration, c. cellular maximal respiration rates, and d. ATP production of SGC-7901 and BGC-823 cells. e. The cellular aerobic glycolysis was evaluated by measures of the extracellular acidification rate (ECAR) from SGC-7901 or BGC-823 cells treated with curcumin (10 μg/ml) following sequential injection (arrow) of glucose (10 mM), oligomycin (1 μM), and 2-DG (100 mM); n = 6. The basal (f) and spared (g) cellular glycolytic rate in SGC-7901 and BGC-823 cells with or without 10 μg/mL curcumin treatment; n = 6. Data are presented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 compared to control or no DMSO group

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