Fig. 5

Chrysin induced Bax activation on mitochondrial. HCC cells were transfected with pORF-HK-2, 24 h later, the cells were treated with 60 μM chrysin for another 24 h, and subcellular fractions were prepared and subjected to SDS-PAGE and probed with indicated protein. a and b, cytosolic Bax, Bak and chrome c expressions in HCC-LM3 cell (a) and SMMC-7721 cell (b) were tested by western blotting. c and d, mitochondrial Bax, Bak, Bcl2, Bcl-xl, chrome c, and HK2 in HCC-LM3 cell (c) and SMMC-7721 cell (d) were tested by western blotting