Table 1 Primers used for the qRT-PCR analysis
genes | Sequence (5’-3’) | |
|---|---|---|
Forward primer | Reverse primer | |
SOX3 | GACCTGTTCGAGAGAACTCATCA | CGGGAAGGGTAGGCTTATCAA |
E-cadherin | CGAGAGCTACACGTTCACGG | GGGTGTCGAGGGAAAAATAGG |
CK-18 | GGCATCCAGAACGAGAAGGAG | ATTGTCCACAGTATTTGCGAAGA |
N-cadherin | TCAGGCGTCTGTAGAGGCTT | ATGCACATCCTTCGATAAGACTG |
Vimentin | GACGCCATCAACACCGAGTT | CTTTGTCGTTGGTTAGCTGGT |
Snail1 | AAGGCCTTCTCTAGGCCCT | CGCAGGTTGGAGCGGTCAG |
Snail2 | TTCGGACCCACACATTACCT | GCAGTGAGGGCAAGAAAAAG |
Twist1 | CAGCTACGCCTTCTCGGTCT | CTGTCCATTTTCTCCTTCTCTGGA |
Zeb1 | GATGATGAATGCGAGTCAGATGC | ACAGCAGTGTCTTGTTGTTGT |
Zeb2 | CAAGAGGCGCAAACAAGCC | GGTTGGCAATACCGTCATCC |
Snail1 promoter | TCAGAAGCGCTCAGACCAC | TTATCTGCCACGCCCCTTT |
Snail1 non-promoter | TGCTCATCTGGGACTCTGTC | GAGGAGAAGGACGAAGGAGC |
β actin | TTGCCGACAGGATGCAGAAG | CAGCGAGGCCAGGATGGAGC |