Fig. 1

Expression of miR-27a in gastric cancer tissues and cells. a Hierarchical cluster heat map of differentially expressed miRNAs in gastric cancer and corresponding normal tissues generated from miRNA sequencing data from the TCGA database. Red in the heat map denotes upregulation; blue denotes downregulation. The green arrow indicates miR-27a. b Upregulation of miR-27a in gastric cancer. P < 0.001.qRT-PCR analysis of miR-27a expression in 50 pairs of gastric cancer and corresponding normal tissues. MiR-27a expression was normalized to U6. c MiR-27a expression in gastric cancers at different clinical stages. P = 0.002. d MiR-27a expression in gastric cancers at different T stages. P < 0.001. e MiR-27a expression in gastric cancers at different N stages. P = 0.026. f MiR-27a expression in gastric cancers at different M stages. P = 0.004. g MiR-27a expression in gastric cancer cell lines (HGC, SGC-7901, AGS, BGC and MGC-803) compared with normal gastric epithelial cells GES-1 detected using qRT-PCR. **P < 0.01 and ***P < 0.001