Fig. 2
MiR-27a affects viability, cell cycle and apoptosis of gastric cancer cells. a QRT-PCR analysis of miR-27a expression in SGC-7901/AGS cells transfected with miR-27a antagomir /miR-27a agomir. b Cell viability assay. SGC-7901 cells transfected with miR-27a antagomir and AGS cells transfected with miR-27a agomir were subjected to MTT assay 24 h after transfection. *P < 0.05, **P < 0.01 and ***P < 0.001. c Effects of miR-27a on colony formation of GC cells. SGC-7901 cells transfected with antagomir and AGS cells transfected with miR-27a agomir were seeded onto 6-well plates. The number of colonies was counted on the 14th day after seeding. Representative micrographs (left) and relative quantification analysis of colonies (right). *P < 0.05 and ***P < 0.001. d Flow cytometric cell apoptosis assay. Histograms depict the proportions of normal, early apoptosis and late apoptosis in SGC-7901/AGS cells transfected with miR-27a antagomir/agomir. *P < 0.05. e Flow cytometric cell cycle distribution assay. Histograms depict the proportions of SGC-7901/AGS cells in G1, S, and G2/M phases after transfection with miR-27a antagomir/agomir. *P < 0.05. f Western blot analysis of Akt, p-Akt, p21, p27 and CyclinD1 expression in SGC-7901 and AGS cells in which miR-27a was upregulated and downregulated, respectively