Fig. 3

MiR-27a affects migration and invasion of gastric cancer cells. a Analysis of migration by wound healing assays. Representative images at 0 and 24 h (right panel). Quantification of the migration area of cells in wound healing assays (left panel). *P < 0.05. b Tumor cell Transwell migration assay. Representative images (up panel) and quantification (down panel) of SGC-7901 and AGS cells migration after 24 h transfection with miR-27a antagomir or miR-27a agomir. **P < 0.01 and ***P < 0.001. c Tumor cell Transwell invasion assay. Representative images (upper panel) and quantification (lower panel) of SGC-7901 and AGS cells invasion after 24 h transfection with miR-27a antagomir or miR-27a agomir. **P < 0.01 and ***P < 0.001. d Western blot analysis of GSK3β, p-GSK3β, Snail, E-cadherin and Vimentin expression in SGC-7901 and AGS cells where miR-27a was downregulated and upregulated, respectively. e Immunofluorescence staining analysis of E-cadherin and Vimentin in SGC-7901 and AGS cells in which miR-27a was downregulated and upregulated, respectively