Fig. 5

PHLPP2 mediates the effects of miR-27a in GC cells. a MTT assay. Cell viability was assessed after PHLPP2 siRNA was transfected into anti-miR-27a SGC-7901 cells. *P < 0.05, **P < 0.01 and ***P < 0.001. b Colony formation assay. Transfection of PHLPP2 siRNA in anti-miR-27a SGC-7901 cells promoted cell colonies formation. **P < 0.01. c Apoptosis assay. Apoptosis analysis via flow cytometry was performed after transfection of PHLPP2 siRNA into the anti-miR-27a SGC-7901 cells. *P < 0.05. d Flow cytometry cell cycle assay. Cell cycle distribution was analyzed after transfection of PHLPP2 siRNA into anti-miR-27a SGC-7901 cells. *P < 0.05. e Analysis of the migration with the wound healing assays. *P < 0.05. f Tumor cell migration assay. Representative images and quantification of migration level in anti-miR-27a SGC-7901 cells 24 h after transfection with PHLLPP2 siRNA. *P < 0.05. g Tumor cell invasion assay. Representative images and quantification of invasion level in anti-miR-27a SGC-7901 cells 24 h after transfection with PHLLPP2 siRNA. *P < 0.05. h Western blot. Expression of PHLPP2 and several key proteins in the Akt/GSK3β pathway was detected after transfection of PHLPP2 siRNA into anti-miR-27a SGC-7901 cells. i Immunofluorescence staining of E-cadherin (green), Vimentin (green) and DAPI (blue) after transfection of PHLPP2 siRNA into anti-miR-27a SGC-7901 cells