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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long non-coding RNA NKILA inhibits migration and invasion of non-small cell lung cancer via NF-κB/Snail pathway

Fig. 5

NKILA negatively regulate IκB phosphorylation and NF-κB activation. (a) Western blotting showing total and phosphorylated IκBα in A549 and H226 cells. Left panel was representative images and right panel was statistical column diagram. (b) Western blot for nuclear and cytoplasm p65 in A549 and H226 cells. GAPDH and Histone 3 (H3) is the loading control for cytoplasm and nuclear, respectively. Left panel was representative images and right panel was statistical column diagram. (c) RIP-derived RNA was measured by qRT-PCR. The levels of qRT-PCR products were expressed as a percentage of input RNA. Data are expressed as means ± SEM. Two-tailed Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001

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