Fig. 3

miR-200a could negatively regulate HMGB1 expression a Online tools including miRcode, miRWalk, miRanda and TargetScan were used to screen out the candidate upstream miRNAs of HMGB1. b The miRNA mimics of the indicated miRNAs were transfected into HepG2 cells, respectively. HMBG1 mRNA expression in response to ectopic miRNA expression of the indicated miRNAs was monitored by using real-time PCR. c and d The protein levels of HMGB1 in HCCLM3 and HepG2 cells in response to miR-200a overexpression or inhibition were determined by using Western blot assays. The data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01