Fig. 2

iASPP induces miR-20a expression in a p53-dependent manner. a qPCR analysis of miR-20a in control or iASPP-silenced HeLa cells and in SiHa cells after transient overexpression of iASPP. b Relative miR-20a expression in 40 paired cancerous and normal tissue samples from CC patients, measured by qPCR analysis. c Protein expression of p53 in CC cells transfected with control or p53 expression vector (p53-vec). d Relative miR-20a expression in CC cells after transient overexpression of p53. e, f ChIP-qPCR assay of p53 occupancy at the human miR-20a and p21 (as a control) loci in control or iASPP-silenced HeLa cells (e), and in SiHa cells following transient overexpression of iASPP (f). **P < 0.01