Fig. 3

RBM38 represses c-Myc expression in breast cancer cells. a, b, e, f MCF-7 and ZR-75-1 cells were transfected with lentivirus to overexpress RBM38 (RBM38) and the control (NC). c-Myc expression significantly decreased at both the protein (a, e) and mRNA levels (b, f) following RBM38 overexpression. c, d, g, h MCF-7 and ZR-75-1 cells were transfected with lentivirus to knockdown RBM38 (sh1, sh2) and the control (SCR). c-Myc expression was significantly upregulated at both the protein (c, g) and mRNA levels (d, h) following RBM38 knockdown. Western blot and qRT-PCR were performed to detect the expression of RBM38 and c-Myc in protein and mRNA level. Results were representative of three independent experiments and presented as the mean ± SEM, *P < 0.05