Fig. 6

Specific c-Myc inhibitors decreases RBM38-induced growth suppression in breast cancer cells. a–f Colony formation assay and CCK-8 assay were performed to explore the growth suppression of RBM38 overexpression cells in the presence of specific c-Myc inhibitors. a–d MCF-7 and ZR-75-1 cells with RBM38 overexpression lentivirus (RBM38) and the control (NC) were treated with 15 nM 10058-F4, 15 nM 10074-G5 respectively. The growth of MCF-7 (a) and ZR-75-1 cells (c) over 21 d was measured using colony formation assays. Quantified data were obtained from colony formation assays in MCF-7 (b) and ZR-75-1 cells (d). The inhibition ratio of colony formation induced by overexpression of RBM38 was calculated by {colony number (NC)-colony number (RBM38)}/colony number (NC). The inhibition ratio of colony formation was significantly decreased by the addition of specific c-Myc inhibitors compared to control cells, respectively, *P < 0.05. e MCF-7 and f ZR-75-1 cells with RBM38 overexpression lentivirus (RBM38) and the control (NC) were treated with 70 nM 10058-F4, 70 nM 10074-G5 respectively over 3 d. The OD value was obtained from CCK-8 assays. The inhibition ratio of proliferation induced by overexpression of RBM38 was calculated by {OD value (NC)-OD value (RBM38)}/OD value (NC). The inhibition ratio of proliferation was significantly reduced in the presence of specific c-Myc inhibitors compared to control cells, *P < 0.05