Fig. 4

AHNAK inhibits TNBC growth and lung metastasis in vivo. MDA-MB-231 or BT549 cells infected with AHNAK or vector lentivirus were injected into the flanks of nude mice. a The growth curves of the tumours are plotted (left: MDA-MB-231; middle: BT549). The weights of the xenograft tumours are summarized in the right panel. All results are expressed as the mean ± SD of three independent experiments, *P < 0.05 and **P < 0.01. b Tumour metastasis in the mouse xenograft model. Metastatic nodules (arrows) on the lung surface. The number of nodules was quantified in the lungs of nude mice (n = 5 per group) 28 days after tail vein injection of AHNAK- or empty vector-transfected MDA-MB-231 and BT549 cells (**, P < 0.01, independent Student’s t-test). c The haematoxylin and eosin stained sections derived from metastatic nodules on the lung surface. Original magnification 100X and 200X