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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: AHNAK suppresses tumour proliferation and invasion by targeting multiple pathways in triple-negative breast cancer

Fig. 5

AHNAK targets the AKT/MAPK signalling pathway and the Wnt/β-catenin pathway. a Relative expression levels of AKT/MAPK signalling proteins were compared between the empty vector- and the AHNAK-expressing MDA-MB-231 and BT549 cells by western blotting. β-actin was used as the loading control. b Image of the blot shows that Wnt activator Wnt3a could reactivate the expression of β-catenin, c-myc, and Wnt1 expression in the MDA-MB-231 and BT549 cells infected with AHNAK-expressing vector. β-actin was used as the loading control. c In contrast to the corresponding vector-transfected groups, the levels of β-catenin, c-myc and Wnt-1 mRNA were significantly decreased in the MDA-MB-231 and the BT549 cells that were transfected with AHNAK-expressing vector. d Relative levels of AKT/MAPK signalling-related proteins were compared between the control and AHNAK siRNA-transfected BT20 and MDA-MB-435 cells by western blotting. e Image of the blot shows that Wnt inhibitor Dkk1 could effectively decrease the expression of β-catenin, c-myc, and wnt1 in the BT20 and MDA-MB-435 cells that were infected with AHNAK siRNA. f In contrast to the control group, the levels of β-catenin, c-myc and Wnt-1 mRNA were significantly increased in the BT20 and MDA-MB-435 cells that were transfected with AHNAK siRNA

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