Fig. 2

Gab2 promotes CRC cell proliferation and colonigenic ability in vitro. a Infected cells were examined by phase contrast microscopy (left panel) and fluorescent microscopy (right panel) and high infection efficiency was seen in these cells under fluorescent microscopy. b After cells were infected with LV-NC and LV-Gab2, or LV-si-Ctrl, LV-Gab2siA, -B or -C, the expression of Gab2 protein was detected by western blot analysis. c The proliferation ability of SW480-NC and SW480-Gab2 cells was detected by CCK-8 assay. d Gab2 overexpression could enhance the colony formation of SW480 cells. e and f The proliferation and colonigenic ability of SW620-si-Ctrl and SW620-Gab2si cells was assessed (two clones, c and d). Columns are the average of three independent experiments ± SEM. *P < 0.05