Fig. 5

Gab2 enhances VEGF expression through ERK/c-Myc pathway. a Western blot analysis of VEGF expression in SW480-NC, SW480-Gab2, SW620-si-Ctrl and SW620-Gab2si cells. b The levels of VEGF mRNA in SW480-NC, SW480-Gab2, SW620-si-Ctrl and SW620-Gab2si cells were detected by qRT-PCR. c Overexpression of Gab2 enhances c-Myc expression and activates ERK1/2, whereas knockdown of Gab2 inhibits c-Myc expression and reduces ERK1/2 activation. d The levels of c-Myc and phosphor‑ERK1/2 were calculated. The data are representative of at least three different experiments ± SEM. *P < 0.05