Fig. 2

LPCAT1 is up-regulated in ccRCC tissues and correlates with the overall survival of patients. a qPCR analyses of LPCAT1-4 in 20 paired ccRCC and normal renal tissues. b Western blot analysis of LPCAT1 in 20 paired ccRCC and normal renal tissues. The upper panel shows the results of three representative paired tissues. N and T represent normal renal and ccRCC tissues, respectively. The lower panel shows the results in 20 paired tissues. GAPDH was used as an internal standard. c Representative IHC results of LPCAT1 expression in low-grade ccRCC tissues, high-grade ccRCC tissues and paired normal renal tissues. Positive LPCAT1 staining were detected in the membrane and cytoplasm of cancerous tissues, while negative LPCAT1 staining were detected in the normal renal tissues. N and T represent normal renal and ccRCC tissues, respectively. d Patients in comparative LPCAT1-high groups had lower overall survival times than those in comparative LPCAT1-low groups (p = 0.018)