Fig. 1

The anticancer gallium compound KP46 is highly active against OS cells. a Full dose response curves for KP46 as compared to the standard OS chemotherapeutics cisplatin and methotrexate were established from 72 h continuous drug exposure assays in the indicated OS cell lines and non-malignant HLF cells as indicated. One out of three independent experiments performed in triplicate is shown. For mean IC₅₀ values derived from all three experiments, compare Table 1. b The impact of low-dose KP46 on the clonogenic potential of the indicated OS cell lines was determined as clonal area (compare Material and Methods). High resolution micrographs of crystal violet stained 24-well plates (upper panel) were taken and evaluated Image J software (lower panel). Data are derived from two independent experiments performed in duplicates. Student’s t-test; ** p < 0.01; *** p < 0.001