Fig. 2

Impact of KP46 on OS cell viability, cell cycle distribution and migratory potential. a, b Induction of apoptotic/necrotic cell death was determined by concomitant staining with HOE/PI as described in Methods. Photomicrographs of HOS cells treated for 24 and 48 h are representatively shown in (a; size bar, 50 μm) and opposed to the microscopical evaluation of two experiments in duplicate for HOS (b) and the other OS cell lines (c) at the indicated times and concentrations of KP46 treatment. Stars directly above the bars define significance compared to the solvent controls, whereas significance between 24 and 48 h of treatment are indicated by brackets. d The effect of a 24 h KP46 exposure on cell cycle distribution in the indicated OS cells was measured by FACS analysis and the percentage of cells in S-phase is shown as mean of three independent experiments. e) Impact of low-dose KP46 on cell migration was measured in transwell filter assay. After a 24 h migration period under KP46 exposure, surviving cells in the lower well were allowed to form colonies in drug-free medium for 7 days. Colonies were fixed and stained at day 10 after start of the migration period. One-way analysis of variance (ANOVA) (b, c) and student’s t-test (d); * p < 0.05; ** p < 0.01; *** p < 0.001