Fig. 1

Co-cultivation with M2 polarized macrophages enhances the resistance of MFC cells to cisplatin. a FCM identification of M2 polarized macrophages derived from murine bone marrow stimulated with IL-4 and IL-13, CD206 was a specific marker for M2. (Un-Mac, unactivated macrophages; M2, macrophages activated by IL-4 and IL-13). b qRT-PCR detection of iNOS, Arg1, IL-10, TGF-β mRNA expression in unactivated and M2 polarized macrophages, GAPDH was assayed as an internal control. (ns p > 0.05, *** p < 0.001). c Cell viability assay of MFC treated with various concentrations of DDP for 24 h, 48 h, and 72 h. d Flow cytometric analyses of apoptotic cells. MFC cells were cultured alone or co-cultured with unactivated (Un-Mac) or M2 polarized macrophages, and then exposed to DDP for 48 h. The quantitative data are presented as the mean ± SD of triplicate experiments. (ns p > 0.05, *** p < 0.001)