Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Exosomal transfer of tumor-associated macrophage-derived miR-21 confers cisplatin resistance in gastric cancer cells

Fig. 4

Exosomal transfer of miR-21 from M2 macrophages to gastric cancer cells. a The top 10 most abundant miRNAs in M2-Exo are listed, and miR-21 was the most abundant. b qRT-PCR detection of miR-21 in MFC, unactivated macrophages (Un-Mac) and M2 polarized macrophages (M2). (**p < 0.01, *** p < 0.001). c qRT-PCR detection of miR-21 in exosomes derived from unactivated macrophages (Un-Mac) and M2 polarized macrophages (M2). (*** p < 0.001). d qRT-PCR detection of miR-21 in MFC cultured alone (ctrl) or co-cultured with M2 macrophages or M2-Exo. (*** p < 0.001). e Exosomes prepared from M2 macrophages transfected with Cy3-labelled miR-21 or without transfection (ctrl) were added to MFC cell cultures. MFC cells were fixed and stained with DAPI and Alexa 488 phalloidin. The fluorescence signals in MFC cells were detected by SP5 confocal microscope. (scale bar, 25um)

Back to article page