Fig. 3From: Benserazide, a dopadecarboxylase inhibitor, suppresses tumor growth by targeting hexokinase 2Western blot assay and siRNA knocking down HK2 expression for CCK-8 assay. a HK2 expressions in various cancer cells. b Quantitative analysis of HK2 expression levels in cancer cells. c The expression of glycolysis-related proteins and apoptosis-associated factors: AMPKα, p-AMPKα, Bcl-2 and Bax. d Quantitative analysis of expression levels of AMPKα, p-AMPKα, Bcl-2 and Bax. e Western blot conforming the suppression of HK2 by siRNA. f Quantitative analysis of HK2 expression levels. g Knocking down HK2 attenuates the sensitivity of SW480 cells to Benz treatment. All data were shown as means ± SEM for three independent experiments. NC, Negative control. h-i Effect of Benz on the proliferation of HCT116 and SW480 cells. CCK8 assay was used to detect the cancer cell proliferation after treatment with different concentrations of the Benz (0, 100, 200, 400 μM) for 24, 48, 72 and 96 h. *P < 0.05, **P < 0.01, ***P < 0.001Back to article page