Fig. 3

DHA inhibits TCTP-dependent cell migration and invasion. a In the migration assays, the TCTP-positive cell lines NOZ, GBC-SD, and OCUG-1, and the TCTP-negative cell lines EH-GB-2 and SGC-996 were pre-treated with either vehicle or DHA (40 μM) for 2 days and then seeded in transwell plates for 24 h. b The relative migration rates are shown in a bar graph. c, d SGC-996 cells were transfected with an empty or TCTP expression vector (c), treated with vehicle or DHA for 2 days, and then seeded in transwell plates for migration assays. The relative migration rates are shown in a bar graph (d). e, f TCTP was depleted in NOZ cells using shRNA (e). The cells were then treated with DHA for 2 days and seeded in transwell plates for migration assays. The relative migration rates are shown in a bar graph (f). The percentage of cells that migrated was scored and normalized to the percentage of migrated vehicle-treated cells. The graphed data represent the mean ± SD of 3 independent experiments. *, p < 0.05; **, p < 0.01, ns: no significant difference (p > 0.05)