Fig. 5

The roles of miR-584-5p and WWP1 in the regulation of gastric cancer cell proliferation and apoptosis. a-c. miR-584-5p up-regulation or WWP1-shRNA inhibits proliferation and induces apoptosis using the cell cycle assay, flow cytometry and cell viability assay. The rescue experiments for miR-584-5p overexpression were performed by ectopic expression of WWP1 without its 3’-UTR in MGC803 cells. d-f). Similar rescue experiments for miR-584-5p silencing was performed by downregulation of WWP1 in SGC7901 cells. g-j. Western blot was used to verify the expression of WWP1. The data came from at least three independent experiments. A representative data set is displayed as mean ± SD values. *p < 0.05, **p < 0.01, ***p < 0.001