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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: FGF2/FGFR1 regulates autophagy in FGFR1-amplified non-small cell lung cancer cells

Fig. 4

Downregulation of beclin-1 prevents autophagy induced by FGFR1 inhibition. a and b H1581 and H520 cells were treated with 1 μM AZD4547 for 24 h. Then, total lysates were harvested and subjected to western blot analysis. c and d H1581 and H520 cells, stably transduced by the indicated lentiviruses (scramble or shFGFR1), were subjected to western blotting analysis. e H1581 cells were transfected with beclin-1 siRNA or control siRNA using Lipofectamine RNAiMAX transfection reagent as described in materials and methods. At 24 h after transfection, the cells were treated with or without AZD4547 (1 μM) for 24 h. f Quantification of LC3-II levels in (e); mean ± SEM, n = 3, ***p < 0.001. g H1581 cells, stably transduced by the indicated lentiviruses (scramble or shFGFR1), were transfected with beclin-1 siRNA or control siRNA, and then cells were subjected to western blotting analysis. h Quantification of LC3-II levels in (g); mean ± SEM, n = 3, ***p < 0.001

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