Fig. 5

Upregulation of beclin-1 by FGFR1 inhibition contributes to induction of autophagy through inhibiting ERK pathway. a H1581 cells were treated with AZD4547 (1 μM) for 24 h prior to treatment with or without FGF2 (25 ng/ml) for 2 h. After treatment, cells were harvested and subjected to western blotting analysis. b H1581 cells, stably transduced by FGFR1 shRNA or control shRNA, were transfected with caMEK1 plasmid or control vector using jetPRIME transfection reagent as described in materials and methods. At 24–48 h after transfection, the cells were harvested and then subjected to western blotting analysis. c H1581 cells were transfected with beclin-1 siRNA or control siRNA using Lipofectamine RNAiMAX transfection reagent. At 24 h after transfection, the cells were treated with or without AZD4547 (1 μM) for 24 h. d H1581 cells, stably transduced by the indicated lentiviruses (scramble or shFGFR1), were transfected with beclin-1 siRNA or control siRNA, and then cells were subjected to western blotting analysis. e MEK phosphorylation was analyzed in a cohort of lung SQCC patients that had altered levels of beclin-1 protein expression using cBioPortal. f MEK phosphorylation was analyzed in a cohort of lung adenocarcinoma patients that had altered levels of beclin-1 expression using TCGA database