Fig. 4

FOXM1 expression in EOC clinical samples and in derived cell lines. a Relative expression of FOXM1b and FOXM1c in paired EOC surgical/effusion samples and derived cell lines, as determined using RT-qPCR with isoform-specific primers and normalized against PPIA. b Expression of FOXM1 mRNA detected by RT-qPCR, normalized to GADPH in siFOXM1, siControl and untransfected (NT) cell lines. c Relative expression of FOXM1b and FOXM1c in primary cell lines after siRNA transfection, as determined using RT-qPCR with isoform-specific primers and normalized against PPIA. d Western blot analysis demonstrating the effectiveness of FOXM1 suppression in EOC cell lines. Data represent mean ± SD. Student’s t-test, *, P ≤ 0.05, **, P ≤ 0.01, ***, P ≤ 0.001, ****, P ≤ 0.0001