Fig. 6

Examining the role of caspases for GAEL-induced cell death. a Viability of A2780-cp cells treated with GAELs alone or pretreated with the pan-caspase inhibitor Q-VD-OPh (QVD) for 4 h, prior to co-treatment with GAELs and QVD for 48 h. Percentage of cells in each quadrant are indicated. b Activated caspases were measured in A2780-cp cells 24 h after initiation of treatment with GLN, MO-101, or cisplatin. Fold caspase activity in comparison to vehicle control treated cells is indicated. Percent viability at the time of cell harvest is indicated as measured by trypan blue exclusion. c Viability of A2780-cp cells treated with GAELs alone or pretreated with the pan-caspase inhibitor Q-VD-OPh (QVD) for 4 h, prior to co-treatment with GAELs and QVD for 48 h. The fold increase in cell viability is shown in co-treated cells, indicated in brackets above the bar. * significantly different from vehicle control; + significantly different from GAEL alone; p < 0.005. d Viability of primary EOC cell samples, EOC126 or EOC146, treated with GAELs alone or pretreated with the pan-caspase inhibitor QVD. No significant difference in cell viability was observed in the presence of QVD, with the exception of the cisplatin positive control. * significantly different from vehicle control; + significantly different from cisplatin alone; p < 0.005