Fig. 7

Effect of AT2R on apoptosis and VEGF expression in bladder tumor xenografts. a apoptosis in tumor sections from bladder tumor xenografts was identified by DeadEnd Colorimetric TUNEL System kit. Two representative phase-contrast micrographs from each treatment condition. The brown-colored nuclei represent TUNEL-positive (apoptotic) cells; b TUNEL-positive cells were quantified as the average of 5 fields selected per tumor (magnification × 200); c levels of VEGF in tumor tissue from bladder tumor xenografts were measured by real-time RT-PCR; d VEGF protein expression was detected by Western blot. Columns, mean from 6 separate experiments; bars, SD. * P <0.01 vs. the control groups