Fig. 1

ERβ1 suppresses cell migration and invasion of AR-positive TNBC cells. a, b Western blot analysis and qRT-PCR of ERβ1 protein and mRNA expression in the control and stable ERβ1-transfected cell lines. c Representative images of wound-healing assays in the control and ERβ1-expressing cell lines. The widths of injury lines made in cells were examined at 0 and 24 h. Wound-healing migration is represented by the widths of injury lines. d Representative results of transwell assays showing the effect of ERβ1 overexpression on the invasion ability in control and ERβ1-expressing MDA-MB-231 and Hs578T cells. *p < 0.05