Fig. 2

ERβ1 suppresses metastasis of AR-positive TNBC by inhibiting ZEB1. a Western blot analysis of E-cadherin and N-cadherin protein in control and ERβ1-expressing cells. GAPDH expression was used as the loading control. b qRT-PCR detection of E-cadherin and N-cadherin mRNA expression in control and ERβ1-expressing MDA-MB-231 and Hs578T cells. c Fluorescent microscopy analysis of the expression of E-cadherin and N-cadherin by immunofluorescence. The red signal represents E-cadherin or N-cadherin protein, and the blue signal represents the nuclear DNA staining by DAPI. d, e Western blot and qRT-PCR analyses of ZEB1, Snail and Twist in the control and ERβ1-expressing cells. f Western blot of E-cadherin and N-cadherin expression after transfection with ZEB1 or empty vector in the control and ERβ1-expressing MDA-MB-231 and Hs578T cells. *p < 0.05