Fig. 5

AR acts as a transcription factor that binds to the promoter of ERβ1. a, b Western blot and qRT-PCR analyses of ERβ1 protein and mRNA with or without AR knockdown in ERβ1-expressing MDA-MB-231 and Hs578T cells in the presence of DHT. c Primers used for PCR in the ChIP assay. d The gel electrophoresis of ChIP assay showed a binding site located in primer 10 without DHT treatment and two binding sites located in primers 2 and 10 with DHT treatment in ERβ1-expressing MDA-MB-231 cells. e, f Western blot and qRT-PCR analyses of AR protein and mRNA in the control and ERβ1-expressing cells. *p < 0.05