Fig. 4
From: In vitro antineoplastic effects of brivaracetam and lacosamide on human glioma cells
Brivaracetam and lacosamide treatments induce epigenetic modification on miR-107 and miR-195 regulatory regions. a-b qRT-PCR of miR-107 a and miR-195-5p b precursors (pre-miRNAs) in U87MG cells upon IC20 LCM or BRV treatments. c Schematic representation of miR-107 gene locus: two different regions of host gene (PANK1) promoter have been analyzed in ChIP assays (P1 and P2). d-e ChIP analysis of acetylated histone H4 and methylated histone H3 occupancy on miR-107 regulatory regions in U87MG cells upon treatment with BRV or LCM at IC20. f Schematic representation of miR-195-5p gene locus: two different regions of host gene (MIR497HG) promoter have been analyzed in ChIP assays (P1 and P2). g-h ChIP analysis of acetylated histone H4 and methylated histone H3 occupancy on miR-195-5p regulatory regions in U87MG cells upon treatment with BRV or LCM at IC20