Fig. 4

PtPT induces apoptosis in a caspase-dependent manner. a A2780 and SKOV3 cells were treated with PtPT at indicating doses for indicating time. PARP and caspase-3,−8,−9 cleavage were detected with western blot. GAPDH was used as a loading control. b A2780 and SKOV3 cells were treated with 6 μM PtPT in the presence or absence of caspase inhibitor z-VAD-fmk (50 μM) for 48 h. PARP and caspase-8 were detected with western blot analyses. GAPDH was used as a loading control. c Cells treated as (b), the PI-positive cells were recorded under an inverted fluorescence microscope. Representative images were shown