Fig. 5

PtPT treatment led to Ub-prs accumulation and ER stress. a and b A2780 and SKOV3 cells were treated with PtPT at indicating doses for indicating time. PARP, Ub-prs and K48-Ub-prs were detected with western blot. GAPDH was used as a loading control. c and d A2780 and SKOV3 cells were treated with PtPT at indicating doses for indicating time. ER stress related proteins ATF4, phosphorylated eIF2α (P-eIF2α), and total eIF2α were detected by western blot analysis. GAPDH was used as a loading control