Fig. 6
PtPT suppressed ovarian tumor xenografts in nude mice. Nude BALB/c mice bearing A2780 and SKOV3 xenografts were randomized to vehicle and PtPT (7.5 mg/kg/day, i.p.) treatment group. Administration of vehicle or PtPT was initiated when the average tumor size reached at 50 mm3 . Tumor size was recorded every other day. Tumor size (a), tumor images (b), tumor weight (c) and body weight (d) were shown. Mean ± SD (n = 6). *P < 0.05, compared with each control. e Total proteins in tumor tissues were extracted and subsequently detected with western blot using PARP, Ub-prs and K48-Ub-prs antibodies. GAPDH was used as a loading control. f Representative images of immunohistochemical staining for Ub-prs, K48-Ub-prs, pro-apoptotic protein Bax, and cleaved caspase-3 in tumor tissues from mice bearing A2780 and SKOV3 tumors treated with either vehicle or PtPT (200 ×)