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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Ibrutinib, a Bruton’s tyrosine kinase inhibitor, exhibits antitumoral activity and induces autophagy in glioblastoma

Fig. 1

Ibrutinib inhibits the proliferation of GBM cells. (a) The concentration- (left) and time- (right) dependent effect of ibrutinib (Ib) on GBM cell viability was assessed using CCK8 assay (the data are presented as the mean ± SEM, n = 4 biological replicates). (b) U87 and LN229 cells were treated with different concentrations of ibrutinib (0, 5, or 10 μM) for 10 day. The formation of cell colonies was then evaluated (the data are presented as the mean ± SEM, n = 3 biological replicates); *p < 0.05 and **p < 0.01 compared with the control group (DMSO). (c) The proliferation capacity of GBM cells treated with ibrutinib (10 μM) for 24 h, as determined by EdU assay. Proliferating cells are stained red and cell nuclei are stained with Hoechst 33,342 (blue); **p < 0.01 compared with the control group (DMSO). (d) The effect of ibrutinib on proliferation of LN229 and U87 cells, as examined by flow cytometry. (e) Western blot analyses of GSK3β, p-Rb, cyclinD1, E2F1, and GAPDH protein levels in LN229 and U87 cells after treatment with different concentrations of ibrutinib for 24 h

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