Fig. 2

In vitro LPS stimulation alters the immuno-phenotype of glioma cells and GSCs. a: After LPS stimulation for 6, 12, or 24 h, the levels of MHC-I, MHC-II, CD80, CD86, TNF-α, IL-6, IL-10, TGF-β1, and TGF-β2 mRNA were examined using real time-PCR. b: After LPS stimulation for 6, 12, or 24 h, the levels of MHC-I, MHC-II, CD80, CD86, TNF-α, IL-6, IL-10, TGF-β1, and TGF-β2 protein in RG2 and U87 GSCs were examined using western blot. c: After LPS stimulation for 6, 12, or 24 h, the levels of TNF-α, IL-6, and IL-10 secretion were examined by ELISA in cell-free supernatants from glioma cells and GSCs. d: After LPS stimulation for 6 h, the expression of MHC-I, MHC-II, CD80 and CD86 were tested using immunofluorescence stain in RG2 GSCs. Representative images are shown. The results are presented as the mean ± SEM of triplicate samples from three independent experiments. *P <0.05, **P <0.01, ***P <0.001