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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: High expression of Collagen Triple Helix Repeat Containing 1 (CTHRC1) facilitates progression of oesophageal squamous cell carcinoma through MAPK/MEK/ERK/FRA-1 activation

Fig. 3

CTHRC1 is critical for ESCC cell proliferation and tumour growth. a, b Knockdown or overexpression efficiency of CTHRC1 in ESCC cells was verified by RT-PCR and western blot. c The proliferation capacity of KYSE510 and KYSE30 cells with depleted CTHRC1 expression and KYSE450 cells with enhanced CTHRC1 expression, as well as vector control cells was measured by CCK8 assay. d, e Representative images of colony formation assays using KYSE510, KYSE30 and KYSE450 cells (left). Columns show the mean number of clones formed in three independent experiments (right). f, g Representative images of tumour formation in nude mice subcutaneously inoculated with shCTHRC1- or vector-KYSE510 cells and CTHRC1- or vector-KYSE450 cells (left), and tumour weights and volumes for the two groups (right). The results are shown as the mean ± SD. The significance of the difference between groups was analysed using two-tailed Student’s t-test. *P < 0.05 and **P < 0.01

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