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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancer

Fig. 2

PPARγ protein expression and the effects of the PPARγ inhibitor GW9662 on TGZ-induced cell death. a Cells (1.75 × 106) were incubated for 24 h, and extracted protein (15 μg) was analyzed by western blot for PPARγ expression. b Cells were pre-cultured for 24 h at a density of 1 × 104 cells/well in 96-well plates and exposed to TGZ (50 μM) with or without GW9662 (5 μM) for 24 h. Cell viability was assessed by fluorescence assay (n = 4–6). Statistical significance was assessed by t-test (TGZ vs. TGZ + GW9662, n.s., not significant). PPARγ, peroxisome proliferator-activated receptor gamma; TGZ, troglitazone

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