Fig. 2From: In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancerPPARĪ³ protein expression and the effects of the PPARĪ³ inhibitor GW9662 on TGZ-induced cell death. a Cells (1.75āĆā106) were incubated for 24Ā h, and extracted protein (15Ā Ī¼g) was analyzed by western blot for PPARĪ³ expression. b Cells were pre-cultured for 24Ā h at a density of 1āĆā104 cells/well in 96-well plates and exposed to TGZ (50Ā Ī¼M) with or without GW9662 (5Ā Ī¼M) for 24Ā h. Cell viability was assessed by fluorescence assay (nā=ā4ā6). Statistical significance was assessed by t-test (TGZ vs. TGZā+āGW9662, n.s., not significant). PPARĪ³, peroxisome proliferator-activated receptor gamma; TGZ, troglitazoneBack to article page