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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancer

Fig. 5

Effects of TGZ on cell invasion and cell migration. a The cell invasion assay was performed using 24-well BD BioCoat™ Matrigel® invasion chambers with 8.0-μm polycarbonate membrane filters. Cells were seeded on membranes at 2 × 105 cells/well with FBS-free medium, after which the membranes were placed into the lower chamber and incubated with 10% FBS-containing medium. After culture with or without TGZ (MIA Paca2: 10 μM, PANC-1: 1 μM) for 24 h, cells on the upper surface of the membranes were removed using a cotton swab. Invasive cells that penetrated through the pores and migrated to the underside of the membrane were stained with Giemsa solution after fixation with 100% methanol. Cell number was quantified under microscopy. b The cell migration assay was conducted in the same manner as the cell invasion assay, except for the use of non-coated chambers. Data represent the mean + S.D. from three or four independent preparations. * p < 0.05 vs. control (t-test). TGZ, troglitazone; FBS, fetal bovine serum

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