Fig. 3

Specificity and affinity of the anti-CLDN1 mAb 6F6. a Reactivity of 10 μg/ml of purified 6F6 mAb towards different CRC cell lines that express or not CLDN1, determined by FACS analysis (Additional file 3). Left: FACS histograms of cells incubated with (gray) or not (dotted line) the 6F6 mAb; Right: quantification of CLDN1 expression by western blotting using the anti-CLDN1 polyclonal antibody JAY8. b Determination of the half saturation binding considered as the apparent Kd. SW620 cells were incubated with increasing concentrations of the 6F6 mAb and binding was assessed by FACS c Biodistribution of ¹²⁵I-labeled 6F6. Images were acquired three days after intravenous injection of 500 μCi of ¹²⁵I-6F6 mAb in the tail vein of mice bearing SW480 or SW480-CLDN1 cell xenografts