Fig. 1

PHF21B is overexpressed in PCa cell lines and tissues. a PHF21B mRNA levels in PCa tissues were assessed by analyzing the TCGA prostate adenocarcinoma mRNA dataset (PRAD, Normal, n = 52; Tumor, n = 497 (Left panel) and 52 paired adjacent non-tumor tissues (Normal) and PCa tissues (Tumor) (Right panel). Data were acquired from the TCGA data portal (https://portal.gdc.cancer.gov/). b PHF21B mRNA levels in PCa tissues were assessed by analyzing the GSE21032 PCa mRNA dataset (Normal prostate tissue (Normal), n = 29; Clinically localized primary PCa tissue (Tumor), n = 131; Data were acquired from the Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/)). c-d Real-time PCR (c) and Western blotting (d) analyses to detect PHF21B expression in normal human prostate epithelial cell (RWPE-1) and cultured PCa cell lines. e-f Real-time PCR (e) and Western blotting (f) analyses of PHF21B expression in paired primary PCa tissues (T) and the matched adjacent non-tumor tissues (ANT) from eight prostate cancer patients (P1-P8). mRNA expression was normalized to GAPDH, and α-tubulin was used as a protein loading control. Data represent the mean ± SD of three independent experiments; *P < 0.05