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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway

Fig. 5

PHF21B activates Wnt/β-catenin signaling pathway. a PHF21B overexpression promoted the stabilization of cytosolic β-catenin, and increased β-catenin nuclear accumulation in indicated cells, as confirmed by western blot analysis. α-Tubulin and P84 were used as loading controls. b Luciferase-reported TCF/LEF transcriptional activity in the indicated cells. c Fold change of indicated genes mRNA expression in Real-time PCR analysis comparing cells overexpressing PHF21B versus Vector (V) or PHF21B-RNAi versus RNAi-vector (RNAi-V). d Knockdown of β-catenin with specific siRNA in PHF21B-overexpressing cells, as confirmed by western blot analysis. e Luciferase-reported TCF/LEF transcriptional activity in the indicated cells. f Histograms showing the mean number of spheres formed by the indicated cells. Each bar represents the mean ± SD of three independent experiments.*P < 0.05

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