Fig. 2
From: CRISPR/Cas9 targeting of GPRC6A suppresses prostate cancer tumorigenesis in a human xenograft model
Editing GPRC6A in PC-3 cells using CRISPR/Cas9. Strategy of targeting GPRC6A in human prostate cancer PC-3 cells using CRISPR/Cas9 and the vector of lentiCRISPR v2 (the figure is adopted from http://genome-engineering.org/gecko/wp-content/uploads/2013/12/lentiCRISPRv2-and-lentiGuide-oligo-cloning-protocol.pdf) (a) and GPRC6A gene structure (b). Blue bars show sgRNA1 and sgRNA3 location in exon 3 of GPRC6A gene. Short guide RNAs (sgRNA) designed to target exon 3 of GPRC6A gene. The controls were empty vector, with no inserts in this region. CRISPR/Cas9 editing in PC-3 human prostate cancer cells decreased GPRC6A protein by Western blot (c) and mRNA by real-time PCR (d). ** Significant difference from control group and sgRNA3 group at p < 0.01 (n ≥ 4)